Luciferase:Article Title: A Leishmania virulence factor harnesses an allosteric kinase switch to regulate its ubiquitin ligase activity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse monoclonal anti-ubiquitin (P4D1) Cell Signaling Technology Cat#3936; RRID: AB_331292 rabbit anti-ubiquitin K48-linkage Boston Biochem Cat#A-101; RRID: AB_2894847 rabbit anti-firefly luciferase Thermo Fisher Scientific Cat#PA5-32209; RRID: AB_2549682 mouse monoclonal anti-His-Tag (27E8) Cell Signaling Technology Cat#2366; RRID: AB_2115719 goat Alexa FluorTM 488-coupled anti-chicken IgY Thermo Fisher Scientific Cat#A-11039; RRID: AB_142924 donkey IRDye 800CW-coupled anti-rabbit IgG LI-COR Biosciences Cat#926-32213; RRID: AB_621848 donkey IRDye 680RD-coupled anti-mouse IgG LI-COR Biosciences Cat#926-68072; RRID: AB_10953628 chicken anti-LmHSP70 Joachim Clos (Hamburg, Germany); Brandau et al. 81 N/A mouse monoclonal anti-PFR1,2 (L13D6) Keith Gull (Oxford, UK); Kohl et al. 82 N/A rabbit anti-TKUL This study N/A Bacterial and virus strains E. coli BL21 (DE3) Thermo Fisher Scientific Cat#C600003 E. coli SoluBL21 Genlantis Cat#C700200 E. coli DH10EMBacY Geneva Biotech N/A E. coli NEBExpress I q NEB Cat#C3037I Biological samples BMDMs from C57BL/6N mice This study N/A Chemicals, peptides, and recombinant proteins Schneider’s insect medium Sigma Aldrich Cat#S9895 RPMI 1640 medium Thermo Fischer Scientific Cat#21875 FCS Anprotec Cat#AC-SM-0189 Penicillin-Streptomycin Sigma Aldrich Cat#P4333 100x nonessential amino acids Sigma Aldrich Cat#M7145 horse serum Cell concepts Cat#S-HUE03-I DMEM without phenol red with 1 g/L glucose and NaHCO 3 Sigma Aldrich Cat#D5921 DMEM GlutaMAX, high glucose (macrophage medium) Gibco Cat#31966-021 DAPI Fluoromount-G mounting medium Southern Biotech Cat#0100-20 Sf-900 III SFM Thermo Fischer Scientific Cat#12658027 ESF-921 Expression Systems Cat#96-001-01 amylose resin NEB Cat#E8021L Lambda protein phosphatase NEB Cat#P0753L TB media CarlRoth GmbH Cat#X972.3 GSH 4B resin Cytiva Cat#17075605 LB media CarlRoth GmbH Cat#X969.3 Protino Ni-NTA agarose Macherey-Nagel Cat#745400.100 Golvatinib Cayman Chemicals Cat#22143 Staurosporine Selleck Chem Cat#S1421 Pro-Q Diamond phosphoprotein stain Invitrogen Cat#P33300 Ub-Prg UbiQ Cat#UbiQ-057 Pierce glutathione magnetic agarose resin Thermo Fisher Scientific Cat#78601 (Continued on next page) Molecular Cell 85, 3711–3728.e1–e11, October 2, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER amylose magnetic beads NEB Cat#E8035S Dynabeads Protein G Invitrogen Cat#10004D Dynabeads Protein A Invitrogen Cat#10002D BS3 (bis(sulfosuccinimidyl)suberate) Thermo Fisher Scientific Cat#A39266 Q5 polymerase NEB Cat#M0491L XtremeGENE HP Sigma Aldrich Cat#06366236001 cOmpleteTM, EDTA-free Protease Inhibitor Cocktail Roche Cat#11873580001 IRDye® 800CW Maleimide LI-COR Cat#929-80020 Morpheus® HT-96 Molecular Dimensions Cat#MD1-47 NativeMark unstained protein standard Invitrogen Cat#11574986 PageRuler plus prestained protein marker Invitrogen Cat#26620 Critical commercial assays SulfoLink kit Thermo Scientific Cat#44999 mNG-Trap Magnetic Agarose Chromotek Cat#ntma Deposited data TKUL KD crystal structure This study PDB: 9GMP IP MS datasets This study Pride: PXD053520 HDX-MS datasets This study Pride: PXD057190 in vitro phospho-MS dataset This study Pride: PXD056904 unprocessed and uncompressed data from Figures 1, 2, 3, 4, 5, 6, 7 and S1–S8, and source data for all graphs This study Mendeley Data: https://doi.org/ 10.17632/528bhp8sgc.1 Experimental models: Organisms/strains L. mexicana MNYC/BZ/62/M379 Sigrid Roberts (Forest Grove, OR, USA); Roberts et al. 83 MNYC/BZ/62/M379 L. mexicana MNYC/BZ/62/M379 Cas9 This study N/A L. mexicana MNYC/BZ/62/M379 ΔTKUL This study N/A L. mexicana MNYC/BZ/62/M379 ΔTKUL + TKUL This study N/A L. mexicana MNYC/BZ/62/M379 ΔTKUL + TKUL K756A This study N/A L. mexicana MNYC/BZ/62/M379 ΔTKUL + TKUL C1392A This study N/A L. mexicana MNYC/BZ/62/M379 TKUL::mNG This study N/A mouse: C57BL/6N mice Charles River Laboratories C57BL/6N Spodoptera frugiperda Sf9 ATCC Cat#CRL-1711 Spodoptera frugiperda Sf21 (IPLB-Sf-21-AE) Oxford Expression Technologies Cat#600105 Trichopulsia Ni Hi5 Invitrogen Cat#10747474 Oligonucleotides table of primers used in this study This study Table S5 Recombinant DNA 4C-His-MBP-TKUL-FL This study N/A 4C-His-MBP-TKUL-ΔN89 This study N/A 4C-His-MBP-TKUL-ΔN119 This study N/A 4C-His-MBP-TKUL-ΔTPR This study N/A 4C-His-MBP-TKUL-FL-K756A This study N/A 4C-His-MBP-TKUL-ΔTPR-K756A This study N/A 4C-His-MBP-TKUL-FL-C1392A This study N/A 4C-His-MBP-TKUL-ΔTPR-C1392A This study N/A 4C-His-MBP-TKUL-FL-T881A This study N/A 4C-His-MBP-TKUL-FL-T883A This study N/A 4C-His-MBP-TKUL-FL-T884A This study N/A (Continued on next page) e2 Molecular Cell 85, 3711–3728.e1–e11, October 2, 2025
Virus:Article Title: A Leishmania virulence factor harnesses an allosteric kinase switch to regulate its ubiquitin ligase activity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse monoclonal anti-ubiquitin (P4D1) Cell Signaling Technology Cat#3936; RRID: AB_331292 rabbit anti-ubiquitin K48-linkage Boston Biochem Cat#A-101; RRID: AB_2894847 rabbit anti-firefly luciferase Thermo Fisher Scientific Cat#PA5-32209; RRID: AB_2549682 mouse monoclonal anti-His-Tag (27E8) Cell Signaling Technology Cat#2366; RRID: AB_2115719 goat Alexa FluorTM 488-coupled anti-chicken IgY Thermo Fisher Scientific Cat#A-11039; RRID: AB_142924 donkey IRDye 800CW-coupled anti-rabbit IgG LI-COR Biosciences Cat#926-32213; RRID: AB_621848 donkey IRDye 680RD-coupled anti-mouse IgG LI-COR Biosciences Cat#926-68072; RRID: AB_10953628 chicken anti-LmHSP70 Joachim Clos (Hamburg, Germany); Brandau et al. 81 N/A mouse monoclonal anti-PFR1,2 (L13D6) Keith Gull (Oxford, UK); Kohl et al. 82 N/A rabbit anti-TKUL This study N/A Bacterial and virus strains E. coli BL21 (DE3) Thermo Fisher Scientific Cat#C600003 E. coli SoluBL21 Genlantis Cat#C700200 E. coli DH10EMBacY Geneva Biotech N/A E. coli NEBExpress I q NEB Cat#C3037I Biological samples BMDMs from C57BL/6N mice This study N/A Chemicals, peptides, and recombinant proteins Schneider’s insect medium Sigma Aldrich Cat#S9895 RPMI 1640 medium Thermo Fischer Scientific Cat#21875 FCS Anprotec Cat#AC-SM-0189 Penicillin-Streptomycin Sigma Aldrich Cat#P4333 100x nonessential amino acids Sigma Aldrich Cat#M7145 horse serum Cell concepts Cat#S-HUE03-I DMEM without phenol red with 1 g/L glucose and NaHCO 3 Sigma Aldrich Cat#D5921 DMEM GlutaMAX, high glucose (macrophage medium) Gibco Cat#31966-021 DAPI Fluoromount-G mounting medium Southern Biotech Cat#0100-20 Sf-900 III SFM Thermo Fischer Scientific Cat#12658027 ESF-921 Expression Systems Cat#96-001-01 amylose resin NEB Cat#E8021L Lambda protein phosphatase NEB Cat#P0753L TB media CarlRoth GmbH Cat#X972.3 GSH 4B resin Cytiva Cat#17075605 LB media CarlRoth GmbH Cat#X969.3 Protino Ni-NTA agarose Macherey-Nagel Cat#745400.100 Golvatinib Cayman Chemicals Cat#22143 Staurosporine Selleck Chem Cat#S1421 Pro-Q Diamond phosphoprotein stain Invitrogen Cat#P33300 Ub-Prg UbiQ Cat#UbiQ-057 Pierce glutathione magnetic agarose resin Thermo Fisher Scientific Cat#78601 (Continued on next page) Molecular Cell 85, 3711–3728.e1–e11, October 2, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER amylose magnetic beads NEB Cat#E8035S Dynabeads Protein G Invitrogen Cat#10004D Dynabeads Protein A Invitrogen Cat#10002D BS3 (bis(sulfosuccinimidyl)suberate) Thermo Fisher Scientific Cat#A39266 Q5 polymerase NEB Cat#M0491L XtremeGENE HP Sigma Aldrich Cat#06366236001 cOmpleteTM, EDTA-free Protease Inhibitor Cocktail Roche Cat#11873580001 IRDye® 800CW Maleimide LI-COR Cat#929-80020 Morpheus® HT-96 Molecular Dimensions Cat#MD1-47 NativeMark unstained protein standard Invitrogen Cat#11574986 PageRuler plus prestained protein marker Invitrogen Cat#26620 Critical commercial assays SulfoLink kit Thermo Scientific Cat#44999 mNG-Trap Magnetic Agarose Chromotek Cat#ntma Deposited data TKUL KD crystal structure This study PDB: 9GMP IP MS datasets This study Pride: PXD053520 HDX-MS datasets This study Pride: PXD057190 in vitro phospho-MS dataset This study Pride: PXD056904 unprocessed and uncompressed data from Figures 1, 2, 3, 4, 5, 6, 7 and S1–S8, and source data for all graphs This study Mendeley Data: https://doi.org/ 10.17632/528bhp8sgc.1 Experimental models: Organisms/strains L. mexicana MNYC/BZ/62/M379 Sigrid Roberts (Forest Grove, OR, USA); Roberts et al. 83 MNYC/BZ/62/M379 L. mexicana MNYC/BZ/62/M379 Cas9 This study N/A L. mexicana MNYC/BZ/62/M379 ΔTKUL This study N/A L. mexicana MNYC/BZ/62/M379 ΔTKUL + TKUL This study N/A L. mexicana MNYC/BZ/62/M379 ΔTKUL + TKUL K756A This study N/A L. mexicana MNYC/BZ/62/M379 ΔTKUL + TKUL C1392A This study N/A L. mexicana MNYC/BZ/62/M379 TKUL::mNG This study N/A mouse: C57BL/6N mice Charles River Laboratories C57BL/6N Spodoptera frugiperda Sf9 ATCC Cat#CRL-1711 Spodoptera frugiperda Sf21 (IPLB-Sf-21-AE) Oxford Expression Technologies Cat#600105 Trichopulsia Ni Hi5 Invitrogen Cat#10747474 Oligonucleotides table of primers used in this study This study Table S5 Recombinant DNA 4C-His-MBP-TKUL-FL This study N/A 4C-His-MBP-TKUL-ΔN89 This study N/A 4C-His-MBP-TKUL-ΔN119 This study N/A 4C-His-MBP-TKUL-ΔTPR This study N/A 4C-His-MBP-TKUL-FL-K756A This study N/A 4C-His-MBP-TKUL-ΔTPR-K756A This study N/A 4C-His-MBP-TKUL-FL-C1392A This study N/A 4C-His-MBP-TKUL-ΔTPR-C1392A This study N/A 4C-His-MBP-TKUL-FL-T881A This study N/A 4C-His-MBP-TKUL-FL-T883A This study N/A 4C-His-MBP-TKUL-FL-T884A This study N/A (Continued on next page) e2 Molecular Cell 85, 3711–3728.e1–e11, October 2, 2025
Recombinant:Article Title: A Leishmania virulence factor harnesses an allosteric kinase switch to regulate its ubiquitin ligase activity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse monoclonal anti-ubiquitin (P4D1) Cell Signaling Technology Cat#3936; RRID: AB_331292 rabbit anti-ubiquitin K48-linkage Boston Biochem Cat#A-101; RRID: AB_2894847 rabbit anti-firefly luciferase Thermo Fisher Scientific Cat#PA5-32209; RRID: AB_2549682 mouse monoclonal anti-His-Tag (27E8) Cell Signaling Technology Cat#2366; RRID: AB_2115719 goat Alexa FluorTM 488-coupled anti-chicken IgY Thermo Fisher Scientific Cat#A-11039; RRID: AB_142924 donkey IRDye 800CW-coupled anti-rabbit IgG LI-COR Biosciences Cat#926-32213; RRID: AB_621848 donkey IRDye 680RD-coupled anti-mouse IgG LI-COR Biosciences Cat#926-68072; RRID: AB_10953628 chicken anti-LmHSP70 Joachim Clos (Hamburg, Germany); Brandau et al. 81 N/A mouse monoclonal anti-PFR1,2 (L13D6) Keith Gull (Oxford, UK); Kohl et al. 82 N/A rabbit anti-TKUL This study N/A Bacterial and virus strains E. coli BL21 (DE3) Thermo Fisher Scientific Cat#C600003 E. coli SoluBL21 Genlantis Cat#C700200 E. coli DH10EMBacY Geneva Biotech N/A E. coli NEBExpress I q NEB Cat#C3037I Biological samples BMDMs from C57BL/6N mice This study N/A Chemicals, peptides, and recombinant proteins Schneider’s insect medium Sigma Aldrich Cat#S9895 RPMI 1640 medium Thermo Fischer Scientific Cat#21875 FCS Anprotec Cat#AC-SM-0189 Penicillin-Streptomycin Sigma Aldrich Cat#P4333 100x nonessential amino acids Sigma Aldrich Cat#M7145 horse serum Cell concepts Cat#S-HUE03-I DMEM without phenol red with 1 g/L glucose and NaHCO 3 Sigma Aldrich Cat#D5921 DMEM GlutaMAX, high glucose (macrophage medium) Gibco Cat#31966-021 DAPI Fluoromount-G mounting medium Southern Biotech Cat#0100-20 Sf-900 III SFM Thermo Fischer Scientific Cat#12658027 ESF-921 Expression Systems Cat#96-001-01 amylose resin NEB Cat#E8021L Lambda protein phosphatase NEB Cat#P0753L TB media CarlRoth GmbH Cat#X972.3 GSH 4B resin Cytiva Cat#17075605 LB media CarlRoth GmbH Cat#X969.3 Protino Ni-NTA agarose Macherey-Nagel Cat#745400.100 Golvatinib Cayman Chemicals Cat#22143 Staurosporine Selleck Chem Cat#S1421 Pro-Q Diamond phosphoprotein stain Invitrogen Cat#P33300 Ub-Prg UbiQ Cat#UbiQ-057 Pierce glutathione magnetic agarose resin Thermo Fisher Scientific Cat#78601 (Continued on next page) Molecular Cell 85, 3711–3728.e1–e11, October 2, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER amylose magnetic beads NEB Cat#E8035S Dynabeads Protein G Invitrogen Cat#10004D Dynabeads Protein A Invitrogen Cat#10002D BS3 (bis(sulfosuccinimidyl)suberate) Thermo Fisher Scientific Cat#A39266 Q5 polymerase NEB Cat#M0491L XtremeGENE HP Sigma Aldrich Cat#06366236001 cOmpleteTM, EDTA-free Protease Inhibitor Cocktail Roche Cat#11873580001 IRDye® 800CW Maleimide LI-COR Cat#929-80020 Morpheus® HT-96 Molecular Dimensions Cat#MD1-47 NativeMark unstained protein standard Invitrogen Cat#11574986 PageRuler plus prestained protein marker Invitrogen Cat#26620 Critical commercial assays SulfoLink kit Thermo Scientific Cat#44999 mNG-Trap Magnetic Agarose Chromotek Cat#ntma Deposited data TKUL KD crystal structure This study PDB: 9GMP IP MS datasets This study Pride: PXD053520 HDX-MS datasets This study Pride: PXD057190 in vitro phospho-MS dataset This study Pride: PXD056904 unprocessed and uncompressed data from Figures 1, 2, 3, 4, 5, 6, 7 and S1–S8, and source data for all graphs This study Mendeley Data: https://doi.org/ 10.17632/528bhp8sgc.1 Experimental models: Organisms/strains L. mexicana MNYC/BZ/62/M379 Sigrid Roberts (Forest Grove, OR, USA); Roberts et al. 83 MNYC/BZ/62/M379 L. mexicana MNYC/BZ/62/M379 Cas9 This study N/A L. mexicana MNYC/BZ/62/M379 ΔTKUL This study N/A L. mexicana MNYC/BZ/62/M379 ΔTKUL + TKUL This study N/A L. mexicana MNYC/BZ/62/M379 ΔTKUL + TKUL K756A This study N/A L. mexicana MNYC/BZ/62/M379 ΔTKUL + TKUL C1392A This study N/A L. mexicana MNYC/BZ/62/M379 TKUL::mNG This study N/A mouse: C57BL/6N mice Charles River Laboratories C57BL/6N Spodoptera frugiperda Sf9 ATCC Cat#CRL-1711 Spodoptera frugiperda Sf21 (IPLB-Sf-21-AE) Oxford Expression Technologies Cat#600105 Trichopulsia Ni Hi5 Invitrogen Cat#10747474 Oligonucleotides table of primers used in this study This study Table S5 Recombinant DNA 4C-His-MBP-TKUL-FL This study N/A 4C-His-MBP-TKUL-ΔN89 This study N/A 4C-His-MBP-TKUL-ΔN119 This study N/A 4C-His-MBP-TKUL-ΔTPR This study N/A 4C-His-MBP-TKUL-FL-K756A This study N/A 4C-His-MBP-TKUL-ΔTPR-K756A This study N/A 4C-His-MBP-TKUL-FL-C1392A This study N/A 4C-His-MBP-TKUL-ΔTPR-C1392A This study N/A 4C-His-MBP-TKUL-FL-T881A This study N/A 4C-His-MBP-TKUL-FL-T883A This study N/A 4C-His-MBP-TKUL-FL-T884A This study N/A (Continued on next page) e2 Molecular Cell 85, 3711–3728.e1–e11, October 2, 2025
Staining:Article Title: A Leishmania virulence factor harnesses an allosteric kinase switch to regulate its ubiquitin ligase activity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse monoclonal anti-ubiquitin (P4D1) Cell Signaling Technology Cat#3936; RRID: AB_331292 rabbit anti-ubiquitin K48-linkage Boston Biochem Cat#A-101; RRID: AB_2894847 rabbit anti-firefly luciferase Thermo Fisher Scientific Cat#PA5-32209; RRID: AB_2549682 mouse monoclonal anti-His-Tag (27E8) Cell Signaling Technology Cat#2366; RRID: AB_2115719 goat Alexa FluorTM 488-coupled anti-chicken IgY Thermo Fisher Scientific Cat#A-11039; RRID: AB_142924 donkey IRDye 800CW-coupled anti-rabbit IgG LI-COR Biosciences Cat#926-32213; RRID: AB_621848 donkey IRDye 680RD-coupled anti-mouse IgG LI-COR Biosciences Cat#926-68072; RRID: AB_10953628 chicken anti-LmHSP70 Joachim Clos (Hamburg, Germany); Brandau et al. 81 N/A mouse monoclonal anti-PFR1,2 (L13D6) Keith Gull (Oxford, UK); Kohl et al. 82 N/A rabbit anti-TKUL This study N/A Bacterial and virus strains E. coli BL21 (DE3) Thermo Fisher Scientific Cat#C600003 E. coli SoluBL21 Genlantis Cat#C700200 E. coli DH10EMBacY Geneva Biotech N/A E. coli NEBExpress I q NEB Cat#C3037I Biological samples BMDMs from C57BL/6N mice This study N/A Chemicals, peptides, and recombinant proteins Schneider’s insect medium Sigma Aldrich Cat#S9895 RPMI 1640 medium Thermo Fischer Scientific Cat#21875 FCS Anprotec Cat#AC-SM-0189 Penicillin-Streptomycin Sigma Aldrich Cat#P4333 100x nonessential amino acids Sigma Aldrich Cat#M7145 horse serum Cell concepts Cat#S-HUE03-I DMEM without phenol red with 1 g/L glucose and NaHCO 3 Sigma Aldrich Cat#D5921 DMEM GlutaMAX, high glucose (macrophage medium) Gibco Cat#31966-021 DAPI Fluoromount-G mounting medium Southern Biotech Cat#0100-20 Sf-900 III SFM Thermo Fischer Scientific Cat#12658027 ESF-921 Expression Systems Cat#96-001-01 amylose resin NEB Cat#E8021L Lambda protein phosphatase NEB Cat#P0753L TB media CarlRoth GmbH Cat#X972.3 GSH 4B resin Cytiva Cat#17075605 LB media CarlRoth GmbH Cat#X969.3 Protino Ni-NTA agarose Macherey-Nagel Cat#745400.100 Golvatinib Cayman Chemicals Cat#22143 Staurosporine Selleck Chem Cat#S1421 Pro-Q Diamond phosphoprotein stain Invitrogen Cat#P33300 Ub-Prg UbiQ Cat#UbiQ-057 Pierce glutathione magnetic agarose resin Thermo Fisher Scientific Cat#78601 (Continued on next page) Molecular Cell 85, 3711–3728.e1–e11, October 2, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER amylose magnetic beads NEB Cat#E8035S Dynabeads Protein G Invitrogen Cat#10004D Dynabeads Protein A Invitrogen Cat#10002D BS3 (bis(sulfosuccinimidyl)suberate) Thermo Fisher Scientific Cat#A39266 Q5 polymerase NEB Cat#M0491L XtremeGENE HP Sigma Aldrich Cat#06366236001 cOmpleteTM, EDTA-free Protease Inhibitor Cocktail Roche Cat#11873580001 IRDye® 800CW Maleimide LI-COR Cat#929-80020 Morpheus® HT-96 Molecular Dimensions Cat#MD1-47 NativeMark unstained protein standard Invitrogen Cat#11574986 PageRuler plus prestained protein marker Invitrogen Cat#26620 Critical commercial assays SulfoLink kit Thermo Scientific Cat#44999 mNG-Trap Magnetic Agarose Chromotek Cat#ntma Deposited data TKUL KD crystal structure This study PDB: 9GMP IP MS datasets This study Pride: PXD053520 HDX-MS datasets This study Pride: PXD057190 in vitro phospho-MS dataset This study Pride: PXD056904 unprocessed and uncompressed data from Figures 1, 2, 3, 4, 5, 6, 7 and S1–S8, and source data for all graphs This study Mendeley Data: https://doi.org/ 10.17632/528bhp8sgc.1 Experimental models: Organisms/strains L. mexicana MNYC/BZ/62/M379 Sigrid Roberts (Forest Grove, OR, USA); Roberts et al. 83 MNYC/BZ/62/M379 L. mexicana MNYC/BZ/62/M379 Cas9 This study N/A L. mexicana MNYC/BZ/62/M379 ΔTKUL This study N/A L. mexicana MNYC/BZ/62/M379 ΔTKUL + TKUL This study N/A L. mexicana MNYC/BZ/62/M379 ΔTKUL + TKUL K756A This study N/A L. mexicana MNYC/BZ/62/M379 ΔTKUL + TKUL C1392A This study N/A L. mexicana MNYC/BZ/62/M379 TKUL::mNG This study N/A mouse: C57BL/6N mice Charles River Laboratories C57BL/6N Spodoptera frugiperda Sf9 ATCC Cat#CRL-1711 Spodoptera frugiperda Sf21 (IPLB-Sf-21-AE) Oxford Expression Technologies Cat#600105 Trichopulsia Ni Hi5 Invitrogen Cat#10747474 Oligonucleotides table of primers used in this study This study Table S5 Recombinant DNA 4C-His-MBP-TKUL-FL This study N/A 4C-His-MBP-TKUL-ΔN89 This study N/A 4C-His-MBP-TKUL-ΔN119 This study N/A 4C-His-MBP-TKUL-ΔTPR This study N/A 4C-His-MBP-TKUL-FL-K756A This study N/A 4C-His-MBP-TKUL-ΔTPR-K756A This study N/A 4C-His-MBP-TKUL-FL-C1392A This study N/A 4C-His-MBP-TKUL-ΔTPR-C1392A This study N/A 4C-His-MBP-TKUL-FL-T881A This study N/A 4C-His-MBP-TKUL-FL-T883A This study N/A 4C-His-MBP-TKUL-FL-T884A This study N/A (Continued on next page) e2 Molecular Cell 85, 3711–3728.e1–e11, October 2, 2025
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